Purification and characterization of embryo-specific soy urease (Glycine max) and its antifungal potential against Paracoccidioides brasiliensis

Ureases are amidohydrolases that catalyze the hydrolysis of urea to ammonia and carbamate. In addition enzymatic function, ureases have fungitoxic insecticidal which independent their catalytic activity. Soy (Glycine max) has two main urease isoforms: ubiquitous embryo-specific, latter is present in beans. view potential applications ureases, this work aimed extract, purify, characterize structure, activity soy against Paracoccidioides brasiliensis. The biochemical characterization was performed, terms optimal pH temperature, as well determination Michaelis�Menten constant (KM) maximum velocity (Vmax). protein sequence identified by mass spectrometry used computational modeling biological structure. optimum temperature enzyme were 6.5 65 �C, respectively, KM 526 mmol L-1 Vmax 7.4 L-1NH3׵gurease-1�s-1 unity a trimer. antifungal assays (in vitro) promising, showing fungicidal profile urease, with minimum inhibitory concentration 10 �g�mL-1. This demonstrated, for first time, embryo-specific Pb18 strain P.